“Phytoplankton and Zooplankton data collected during the Black Sea cruises of UkrSCES R/V "Georgyi Ushakov" in 1992”

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Kovalishina S. (2016): “Phytoplankton and Zooplankton data collected during the Black Sea cruises of UkrSCES R/V "Georgyi Ushakov" in 1992”. v1.1. Ukrainian Scientific Centre of Ecology of the Sea (UkrSCES). Dataset/Occurrence. http://gp.sea.gov.ua:8082/ipt/resource?r=georgy_ushakov_1992&v=1.1

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此資料的發布者及權利單位為 Ukrainian Scientific Centre of Ecology of the Sea (UkrSCES)。 This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC) 4.0 License.

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此資源已向GBIF註冊，並指定以下之GBIF UUID: e8d1d94b-30b0-4278-aaa4-de9f78723dac。  Ukrainian Scientific Centre of Ecology of the Sea (UkrSCES) 發佈此資源，並經由Ocean Biodiversity Information System同意向GBIF註冊成為資料發佈者。

關鍵字

Occurrence; Zooplankton; Phytoplankton; R/V "Georgyi Ushakov"; Black sea; monitoring; cruise; Observation; Observation; monitoring; Black sea; Zooplankton; Phytoplankton

聯絡資訊

地理涵蓋範圍

Cruises carried out in the Black Sea in accordance with the "Program of expeditionary researches of the Black sea" within the "Plan for the scientific reserche expeditions of the Ministry of Ecology, Ukraine on 1992-1993".

時間涵蓋範圍

取樣方法

Cruise duration of 22 days (17. 03.-07.04.1992) was performed in one stage. Transition from the Odessa port to ecological station number 100 at the ship time 7-9 o'clock in the morning (within the integrated global ocean station system (IGOSS) program). Expedition works were carried out on 13 meridional cuts with the complex supervision at ecological stations 1st and 2nd categories, and at three daily stations. Arrival of the vessel at station of 1st category is obligatory by 7-9 o'clock ship time, duration of works at station of 1st category till 6 hours, 2nd categories - till 2,5 hours. After completion of works at the stations the vessel arrives to the main port. The next activities were carried out in the upper layers of the seawater (0-200 m), from 08:00 to 10:00 on the 1st category ecological stations: sampling of phytoplankton and zooplankton; primary production, biomass, phytoplankton and zooplankton size and species composition determination; total number of bacterial biomass and time rate carbon dioxide assimilation determination; photosynthetic pigment determination; bottom sediments and benthos sampling. On the 2nd category ecological stations were performed the next hydrobiological observations: phytoplankton and zooplankton sampling; bottom sediments and benthos sampling; total number of bacterial biomass determination. Coastal station was sampled to determine the bacterial contamination of the marine water surface.

方法步驟描述:

1. When working on the definition of qualitative and quantitative composition of phytoplankton, of the photosynthetic pigments and the primary production the next equipment was used: Spectrophotometer SF-46 LOMO; vacuum pump; laboratory centrifuges; bottles of a tungsten glass volume of 230 ml; membrane filters "SYNPOR" with a pore diameter of 0.6-0.9 mсm; bunsen flask volume of 5 liters; "BIOLAM" microscope; camera for counting the number of phytoplankton cells, volume of 0.1 ml; installation for filtration; flowing aquarium-incubator; light filters 46, 25, 10, 1% of the surface illumination; batchers volume of 0.05 ml, 0.1 ml; electric stove with an adjustable heating temperature; plastic bathometers "NISKIN" with a volume of 30 liters. Water sampling was carried out using a stationary ship winch "LER0K-1.2" on the starboard side at the bow. Laboratory processing of samples was carried out in the premises № 65 and № 297 of the ship. To conduct work on the definition of qualitative and quantitative characteristics of zooplankton and zoobenthos the next devices and equipment have been used: Plankton net of Nansen; large net of "Djedy"; binocular microscope MBS-9; camera for counting zooplankton volume of 3.5 ml, and 4.5 ml; installation thickening samples; grab “Ocean-0.25”. Sampling of zooplankton produced on the upper deck at the stern using winch "LEROK-1.2". In conducting microbiological work used the next equipment: Drying locker 2 B – 151; aquarium-incubator “RAKKA SKONE” thermostat TS-80-M-2; electric stove; filtration plant; membrane filters "VLADIPOR" with a pore diameter of 0.35 mсm. During the expedition cruise 60-A the stations 1st and 2nd category were sampled. In all stations of the 1st and 2nd category, the discharge of zooplankton samples were taken from depths 0-10, 10-25, 25-50, 50-100, 100-200 m. Sampling for the quantitative analysis of macrobenthos was carried out at the stations with a depth of less than 200 m. At all 2nd category stations phytoplankton sampling produced on the layers 0, 10, 25, 50 and 100 m, in layer 0 m determined by bacterial counts. To evaluate the bacterial contamination of surface waters at the stations 20, 21, 22, 23, 39, 40, 41, 42, 53, 65, 66 was determined Escherichia coli index. To determine the qualitative and quantitative composition of phytoplankton, photosynthetic pigments, and bacterioplankton indicators at the stations of the 2nd category layers were chosen depending on the layer transparency. Selected an appropriate layers 100%, 46%, 25%, 10% and 1% of surface illumination. A layer of photosynthesis and choice layers for sampling have been calculated on the basis of transparency, according to the standard formula: Z = 1.3*D*(2-LgIz) Z – depth in meters D – transparency in meters Iz – light transmission filter in % During the 60-A Cruise selection and fixation of phytoplankton samples was performed according to standard methods. Samples of zooplankton were carried out by using a large net of Djedy, with a diameter of 37 cm inlet filter and a filtering gas cone. Net samples were collected by the standard technique, fiberwise to a depth of 200 meters. large comb jelly and jellyfish were removed from the samples, and were counted and measured separately. The sample was fixed with formalin and marked. To determine the total number of microbial biomass, water volume of 3-20 ml was filtered through a membrane filter of 1 cm². Microorganisms deposited on the filter were fixed in formalin vapors and preserved for future work in the conditions of the coastal laboratories. Number of coliform bacteria was determined by the method of membrane filters. The water volume of 10-100 ml was passed through a filter with a pore diameter of 0.35 microns, which is superimposed on Endo Agar. All organisms that create dark colonies with a metallic sheen during 24 hours incubation at 37 ° C were allocated to the group coliform. Production of bacterial biomass was determined by the radiocarbon method. Tackling zoobenthos sampled by bottom grab "Ocean-0.25" with an area capture of 0.25 m². The descent and ascent of the bottom grab carried out at a speed of 1 m / s. Soil samples were washed through a nylon bag with a mesh size of 1 mm. The collected samples of macrobenthos partially treated in the ship's laboratory according to the standard method. The rest of the samples were recorded and are fully prepared for processing in a coastal laboratory. During the cruise next data was selected and processed: 240 seawater samples from 67 stations to determine the qualitative and quantitative characteristics of phytoplankton; 340 set samples from 67 stations to determine the qualitative and quantitative characteristics of zooplankton; 85 seawater samples from 17 stations to determine the primary production; 85 seawater samples from 17 stations to determine the concentrations photosynthetic pigment (chlorophyll "A", "B", "C" and pheophytin); 30 soil samples from 30 stations to determine the qualitative and quantitative characteristics of macro benthos; 150 seawater samples from 67 stations to determine the total number and biomass of microorganisms; 80 seawater samples from 16 stations to measure the value of the bacterial biomass product.

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